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ABU HAMMAD, Khalil, DINU, Vlad, MACCALMAN, Thomas E., PATTEM, Jacob, GOODALL, Margaret, GILLIS, Richard B, JEFFERIS, Roy and HARDING, Stephen E. (2023). Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid. European biophysics journal : EBJ, 52 (4-5), 439-443.
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Abstract
The solution properties of two different glycoforms of IgG1 (IgG1Cri and IgG1Wid) are compared using primarily sedimentation equilibrium analysis with two complementary analysis routines: SEDFIT-MSTAR and MULTISIG. IgGCri bears diantennary complex-type glycans on its Fc domain that are fully core fucosylated and partially sialylated, whilst on IgGWid, they are non-fucosylated, partially galactosylated and non-sialylated. IgGWid is also Fab glycosylated. Despite these differences, SEDFIT-MSTAR analysis shows similar weight average molar masses Mw of ~ (150 ± 5) kDa for IgGCri and ~ (154 ± 5) kDa for IgGWid and both glycoforms show evidence of the presence of a small fraction of dimer confirmed by MULTISIG analysis and also by sedimentation coefficient distributions from supportive sedimentation velocity measurements. The closeness of the sedimentation equilibrium behaviour and sedimentation coefficient distributions with a main peak sedimentation coefficient of ~ 6.4S for both glycoforms at different concentrations suggest that the different glycosylation profiles do not significantly impact on molar mass (molecular weight) nor conformation in solution.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Polysaccharides; Immunoglobulin G; Glycosylation; Physical Phenomena; SEDFIT-MSTAR; MULTISIG; Fc/Fab glycans; c(M)-distribution; c(s)-distribution; Fc/Fab glycans; MULTISIG; SEDFIT-MSTAR; c(M)-distribution; c(s)-distribution; Glycosylation; Immunoglobulin G; Physical Phenomena; Polysaccharides; 0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics; 0299 Other Physical Sciences; 0601 Biochemistry and Cell Biology; Biophysics; 3101 Biochemistry and cell biology; 5105 Medical and biological physics |
| Identification Number: | https://doi.org/10.1007/s00249-023-01656-x |
| Page Range: | 439-443 |
| SWORD Depositor: | Symplectic Elements |
| Depositing User: | Symplectic Elements |
| Date Deposited: | 18 Sep 2023 10:22 |
| Last Modified: | 11 Oct 2023 11:46 |
| URI: | https://shura.shu.ac.uk/id/eprint/32402 |
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