HOYLAND, J. A., LE MAITRE, C. and FREEMONT, A. J. (2008). Investigation of the role of IL-1 and TNF in matrix degradation in the intervertebral disc. Rheumatology, 47 (6), 809-814.Full text not available from this repository.
Objective. To establish if IL-1 or TNF regulates matrix degradation in the non-degenerate or degenerate intervertebral disc (IVD).Methods. In situ zymography (ISZ) has been used to investigate the role of IL-1 and TNF in the matrix degradation characterizing symptomatic IVD degeneration. ISZ employed three substrates (gelatin, collagen II, casein) and four different challenges, IL-1β, IL-1 receptor antagonist (IL-1Ra), TNF-α and anti-TNF. Results. We have shown for the first time that whilst IL-1β will stimulate and IL-1 receptor antagonist will inhibit matrix degradation in intact human IVD tissue, neither TNF-α nor anti-TNF have any measurable effect on degradation of these matrices. Conclusion. This study has addressed a current area of controversy in IVD biology, namely, whether either IL-1 or TNF or both are involved in driving matrix degradation. Our data indicate that IL-1 is a key cytokine mediating matrix degradation in the IVD and therefore a therapeutic target.
|Research Institute, Centre or Group:||Biomolecular Sciences Research Centre|
|Depositing User:||Sarah Ward|
|Date Deposited:||23 Feb 2011 16:01|
|Last Modified:||23 Feb 2011 16:01|
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