Investigation of the role of IL-1 and TNF in matrix degradation in the intervertebral disc

HOYLAND, J. A., LE MAITRE, C. and FREEMONT, A. J. (2008). Investigation of the role of IL-1 and TNF in matrix degradation in the intervertebral disc. Rheumatology, 47 (6), 809-814.

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Abstract

Objective. To establish if IL-1 or TNF regulates matrix degradation in the non-degenerate or degenerate intervertebral disc (IVD).

Methods. In situ zymography (ISZ) has been used to investigate the role of IL-1 and TNF in the matrix degradation characterizing symptomatic IVD degeneration. ISZ employed three substrates (gelatin, collagen II, casein) and four different challenges, IL-1β, IL-1 receptor antagonist (IL-1Ra), TNF-α and anti-TNF.

Results. We have shown for the first time that whilst IL-1β will stimulate and IL-1 receptor antagonist will inhibit matrix degradation in intact human IVD tissue, neither TNF-α nor anti-TNF have any measurable effect on degradation of these matrices.

Conclusion. This study has addressed a current area of controversy in IVD biology, namely, whether either IL-1 or TNF or both are involved in driving matrix degradation. Our data indicate that IL-1 is a key cytokine mediating matrix degradation in the IVD and therefore a therapeutic target.

Item Type:	Article
Research Institute, Centre or Group:	Biomedical Research Centre
Identification Number:	10.1093/rheumatology/ken056
Depositing User:	Sarah Ward
Date Deposited:	23 Feb 2011 16:01
Last Modified:	23 Feb 2011 16:01
URI:	http://shura.shu.ac.uk/id/eprint/3224

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