Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin

CHUN, Taewoo, PATTEM, Jacob, GILLIS, Richard, DINU, Vlad T., YAKUBOV, Gleb E., CORFIELD, Anthony P. and HARDING, Stephen E. (2023). Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin. Scientific Reports, 13 (1): 11367.

Preview	PDF 41598_2023_Article_38036.pdf - Published Version Creative Commons Attribution. Download (1MB) | Preview
Preview	PDF 41598_2023_38036_MOESM2_ESM.pdf - Supplemental Material Creative Commons Attribution. Download (1MB) | Preview
	Microsoft Excel 41598_2023_38036_MOESM1_ESM.xlsx - Supplemental Material Creative Commons Attribution. Download (21kB)

Abstract

Glycopeptide antibiotics are regularly used in ophthalmology to treat infections of Gram-positive bacteria. Aggregative interactions of antibiotics with mucins however can lead to long exposure and increases the risk of resistant species. This study focuses on the evaluation of potential interactions of the last line of defence glycopeptide antibiotic teicoplanin with an ocular mucin model using precision matrix free hydrodynamic and microscopic techniques: sedimentation velocity in the analytical ultracentrifuge (SV-AUC), dynamic light scattering (DLS) and atomic force microscopy (AFM). For the mixtures of teicoplanin at higher doses (1.25 mg/mL and 12.5 mg/mL), it was shown to interact and aggregate with bovine submaxillary mucin (BSM) in the distributions of both sedimentation coefficients by SV-AUC and hydrodynamic radii by DLS. The presence of aggregates was confirmed by AFM for higher concentrations. We suggest that teicoplanin eye drop formulations should be delivered at concentrations of < 1.25 mg/mL to avoid potentially harmful aggregations.

Item Type:	Article
Additional Information:	** From Springer Nature via Jisc Publications Router ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ ** Acknowledgements: We thank Dr M.K. Phillips-Jones for originating this study’s idea and contributing to constructive discussions. This work was supported, in part, by the UK Biotechnology and Biological Sciences Research Council [grant number BB/T006404/1]. **Journal IDs: eissn 2045-2322 **Article IDs: publisher-id: s41598-023-38036-6; manuscript: 38036 **History: collection 12-2023; online 13-07-2023; published_online 13-07-2023; accepted 01-07-2023; registration 01-07-2023; submitted 05-04-2023
Identification Number:	https://doi.org/10.1038/s41598-023-38036-6
SWORD Depositor:	Colin Knott
Depositing User:	Colin Knott
Date Deposited:	17 Jul 2023 09:46
Last Modified:	11 Oct 2023 13:30
URI:	https://shura.shu.ac.uk/id/eprint/32139

Actions (login required)

View Item

Downloads

Publication Metrics