OOI, N., MILLER, K., HOBBS, J., RHYS-WILLIAMS, W., LOVE, W. and CHOPRA, I. (2009). XF-73, a novel antistaphylococcal membrane-active agent with rapid bactericidal activity. Journal of Antimicrobial Chemotherapy, 64 (4), 735-740.Full text not available from this repository.
Objectives XF-73 is a novel porphyrin antibacterial agent previously reported to inhibit a range of Gram-positive bacterial species, including Staphylococcus aureus. Its mode of action is unknown. Using S. aureus as a model organism we sought to examine the basis of its antibacterial activity.Methods The effects of XF-73 on the growth and survival of S. aureus SH1000 were investigated by viable count and culture absorbance techniques. Inhibition of macromolecular synthesis and disruption of membrane integrity after exposure to XF-73 were examined by radiolabelling experiments, the BacLight fluorescent dye assay and measurement of K+ and ATP leakage from the cell. The effect of XF-73 on a staphylococcal coupled transcription–translation system was also investigated. Results XF-73 was rapidly bactericidal against S. aureus SH1000 and demonstrated more rapid killing kinetics than all other comparator agents when tested at an equivalent multiple (4×) of the MIC. Exposure of S. aureus to XF-73 for 10 min completely inhibited DNA, RNA and protein synthesis. XF-73 had no effect on transcription and translation in vitro. Cells exposed to XF-73 gave a positive response in the BacLight assay, which detects membrane damage. The drug also caused substantial loss of K+ and ATP from the cell, but did not promote bacterial lysis. Conclusions XF-73 exhibited rapid membrane-perturbing activity, which is likely to be responsible for inhibition of macromolecular synthesis and the death of staphylococci exposed to the drug.
|Research Institute, Centre or Group:||Biomolecular Sciences Research Centre|
|Depositing User:||Sarah Ward|
|Date Deposited:||15 Feb 2011 12:24|
|Last Modified:||15 Feb 2011 12:24|
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