CHUN, T., PATTEM, J, GILLIS, Richard, DINU, V.T., YAKUBOV, G.E., CORFIELD, A.P. and HARDING, S. (2022). Self-Association of the glycan antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics. [Pre-print] (Unpublished)
Preprints have not been peer-reviewed. They should not be relied on to guide clinical practice or health related behaviour and should not be regarded as conclusive or be reported in news media as established information.
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v1_covered.pdf - Pre-print
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v1_covered.pdf - Pre-print
Available under License Creative Commons Attribution.
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Abstract
The semi-synthetic glycan antibiotic teicoplanin is used for the treatment of serious Gram-positive related bacterial infections and can be administered intravenously, intramuscularly, topically (ocular infections), or orally. It has also been considered for targeting viral infection by SARS-CoV-2. The hydrodynamic properties of teicoplanin A2 (monomer molar mass ~ 1880 g/mol) were examined in phosphate chloride buffer (pH 6.8, I = 0.10 M) using sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge together with capillary (rolling ball) viscometry. In the concentration range, 0-10 mg/mL teicoplanin A2 was found to self-associate plateauing > 1 mg/mL to give a molar mass of (35400 ± 1000) g/mol corresponding to ~ (19 ± 1) mers, with a sedimentation coefficient s20,w = ~ 4.65 S. The intrinsic viscosity [h] was found to be (3.2 ± 0.1) mL/g: both this, the value for s20,w and the hydrodynamic radius from dynamic light scattering is consistent with a globular macromolecular assembly, with a swelling ratio through dynamic hydration processes of ~2.
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