In depth investigation of the capabilities and limitations of combined proteomic-MALDI MS based approach for the forensic detection of blood

KENNEDY, Katie, GANNICLIFFE, Chris, COLE, Laura, SEALEY, Mark and FRANCESE, Simona (2022). In depth investigation of the capabilities and limitations of combined proteomic-MALDI MS based approach for the forensic detection of blood. Science & Justice, 62 (5), 602-609.

[img]
Preview
PDF
Francese-InDepthInvestigationOfTheCapabilities(VoR).pdf - Published Version
Creative Commons Attribution.

Download (863kB) | Preview
Official URL: https://www.sciencedirect.com/science/article/pii/...
Open Access URL: https://doi.org/10.1016/j.scijus.2022.09.001 (Published version)
Link to published version:: https://doi.org/10.1016/j.scijus.2022.09.001
Related URLs:

    Abstract

    For the past 7 years, Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS) based methods have been developed and published for the forensic detection of blood in stains and fingermarks. However, in the view of adoption in an operational context, further investigation into the capabilities and limitations of this approach must be conducted. The refinement and testing of this approach must also be tailored to the requirements of the end users, enabling them to address the specific circumstances most encountered in a forensic scenario. The present study delves deeper into the assessment of the applicability of MALDI MS based strategy for the reliable and robust detection of human blood through: (i) a semi-qualitative assessment of the sensitivity of the method, (ii) a wider investigation of the compatibility of the method with the prior application of commonly used presumptive tests and (iii) assessment of the specificity of the method (when blood is present in mixture with other biofluids) and of its robustness, by assessing blood detection from a range of porous materials. The findings strengthen the evidence supporting the adoption of MALDI MS based approaches as a confirmatory test for the forensic detection of human blood in an operational context.

    Item Type: Article
    Uncontrolled Keywords: Blood; Forensic; Validation; MALDI; Compatibility; Biofluids; Legal & Forensic Medicine
    Identification Number: https://doi.org/10.1016/j.scijus.2022.09.001
    Page Range: 602-609
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 25 Oct 2022 16:20
    Last Modified: 25 Oct 2022 16:20
    URI: https://shura.shu.ac.uk/id/eprint/30941

    Actions (login required)

    View Item View Item

    Downloads

    Downloads per month over past year

    View more statistics