Seeing beyond the limit: a guide to choosing the right super-resolution microscopy technique

VALLI, J., GARCIA-BURGOS, A., ROONEY, L.M., DE MELO E OLIVEIRA, B.V., DUNCAN, Rory and RICKMAN, C. (2021). Seeing beyond the limit: a guide to choosing the right super-resolution microscopy technique. Journal of Biological Chemistry, 297 (1): 100791.

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Official URL: https://www.sciencedirect.com/science/article/pii/...
Open Access URL: https://doi.org/10.1016/j.jbc.2021.100791 (Published version)
Link to published version:: https://doi.org/10.1016/j.jbc.2021.100791
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    Abstract

    Super-resolution microscopy has become an increasingly popular and robust tool across the life sciences to study minute cellular structures and processes. However, with the increasing number of available super-resolution techniques has come an increased complexity and burden of choice in planning imaging experiments. Choosing the right super-resolution technique to answer a given biological question is vital for understanding and interpreting biological relevance. This is an often-neglected and complex task that should take into account well-defined criteria (e.g., sample type, structure size, imaging requirements). Trade-offs in different imaging capabilities are inevitable; thus, many researchers still find it challenging to select the most suitable technique that will best answer their biological question. This review aims to provide an overview and clarify the concepts underlying the most commonly available super-resolution techniques as well as guide researchers through all aspects that should be considered before opting for a given technique.

    Item Type: Article
    Uncontrolled Keywords: diffraction limit; fluorescence; imaging; localization; microscopy; molecular dynamics; molecular imaging; protein–protein interactions; super resolution; Animals; Cell Survival; Fluorescent Dyes; Humans; Microscopy; Molecular Dynamics Simulation; Animals; Humans; Fluorescent Dyes; Microscopy; Cell Survival; Molecular Dynamics Simulation; 03 Chemical Sciences; 06 Biological Sciences; 11 Medical and Health Sciences; Biochemistry & Molecular Biology
    Identification Number: https://doi.org/10.1016/j.jbc.2021.100791
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 09 Sep 2022 09:10
    Last Modified: 09 Sep 2022 09:10
    URI: http://shura.shu.ac.uk/id/eprint/30537

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