Spectroscopic Ellipsometry Detection of Prostate Cancer Bio-Marker PCA3 Using Specific Non-Labeled Aptamer: Comparison with Electrochemical Detection

TAKITA, Sarra, NABOK, Alexei, SMITH, David and LISHCHUK, Anna (2021). Spectroscopic Ellipsometry Detection of Prostate Cancer Bio-Marker PCA3 Using Specific Non-Labeled Aptamer: Comparison with Electrochemical Detection. Chemistry Proceedings, 5 (1), p. 65.

[img]
Preview
PDF
chemproc-05-00065.pdf - Published Version
Creative Commons Attribution.

Download (1MB) | Preview
Official URL: https://www.mdpi.com/2673-4583/5/1/65
Open Access URL: https://www.mdpi.com/2673-4583/5/1/65/pdf (Published version)
Link to published version:: https://doi.org/10.3390/csac2021-10453
Related URLs:

    Abstract

    <jats:p>The most common prostate cancer (PCa) diagnostics, which are based on detection of prostate-specific antigens (PSA) in blood, have specificity limitations often resulting in both false-positive and false-negative results; therefore, improvement in PCa diagnostics using more specific PCa biomarkers is of high importance. Studies have shown that the long noncoding RNA Prostate Cancer Antigen 3 (lncPCA3) that is over-expressed in the urine of prostate cancer patients is an ideal biomarker for non-invasive early diagnostics of PCa. Geno-sensors based on aptamer bioreceptors (aptasensors) offer cost- and time-effective, and precise diagnostic tools for detecting PCa biomarkers. In this study, we report on further developments of RNA-based aptasensors exploiting optical (spectroscopic ellipsometry) measurements in comparison with electrochemical (CV and IS) measurements published earlier. These sensors were made by immobilization of thiolated CG-3 RNA aptamers on the surface of gold. Instead of a redox-labelled aptamer used previously in electrochemical measurements, a non-labelled aptamer was used here in a combination with total internal reflection ellipsometry (TIRE) measurements. The results obtained by these two methods were compared. The method of TIRE is potentially highly sensitive and comparable in that respect with electrochemical methods capable of detection of PCA3 in sub-pM levels of concentration. The required selectivity is provided by the high affinity of PCA3-to-aptamer binding with KD in the 10−9 M range. The spectroscopic ellipsometry measurements provided additional information on the processes of PCA3 to aptamer binding.</jats:p>

    Item Type: Article
    Identification Number: https://doi.org/10.3390/csac2021-10453
    Page Range: p. 65
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 04 Jan 2022 14:58
    Last Modified: 04 Jan 2022 15:00
    URI: http://shura.shu.ac.uk/id/eprint/29504

    Actions (login required)

    View Item View Item

    Downloads

    Downloads per month over past year

    View more statistics