Comparison of Osteosarcoma Aggregated Tumour Models with Human Tissue by Multimodal Mass Spectrometry Imaging

FLINT, Lucy E., HAMM, Gregory, READY, Joseph D., LING, Stephanie, DUCKETT, Catherine J., CROSS, Neil A., COLE, Laura M., SMITH, David P., GOODWIN, Richard J. A. and CLENCH, Malcolm R. (2021). Comparison of Osteosarcoma Aggregated Tumour Models with Human Tissue by Multimodal Mass Spectrometry Imaging. Metabolites, 11 (8).

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Official URL: https://www.mdpi.com/2218-1989/11/8/506
Open Access URL: https://www.mdpi.com/2218-1989/11/8/506/pdf (Published version)
Link to published version:: https://doi.org/10.3390/metabo11080506
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    Abstract

    Osteosarcoma (OS) is the most common primary bone malignancy and largely effects adolescents and young adults, with 60% of patients under the age of 25. There are multiple cell models of OS described in vitro that express the specific genetic alterations of the sarcoma. In the work reported here, multiple mass spectrometry imaging (MSI) modalities were employed to characterise two aggregated cellular models of OS models formed using the MG63 and SAOS-2 cell lines. Phenotyping of the metabolite activity within the two OS aggregoid models was achieved and a comparison of the metabolite data with OS human tissue samples revealed relevant fatty acid and phospholipid markers. Although, annotations of these species require MS/MS analysis for confident identification of the metabolites. From the putative assignments however, it was suggested that the MG63 aggregoids are an aggressive tumour model that exhibited metastatic-like potential. Alternatively, the SAOS-2 aggregoids are more mature osteoblast-like phenotype that expressed characteristics of cellular differentiation and bone development. It was determined the two OS aggregoid models shared similarities of metabolic behaviour with different regions of OS human tissues, specifically of the higher metastatic grade.

    Item Type: Article
    Additional Information: ** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2218-1989 **History: published 31-07-2021; accepted 30-07-2021
    Uncontrolled Keywords: osteosarcoma, DESI, mass spectrometry imaging, LA-ICP-MS, metabolomics, imaging mass cytometry
    Identification Number: https://doi.org/10.3390/metabo11080506
    SWORD Depositor: Colin Knott
    Depositing User: Colin Knott
    Date Deposited: 02 Aug 2021 11:18
    Last Modified: 02 Aug 2021 11:30
    URI: http://shura.shu.ac.uk/id/eprint/28898

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