Influence of probiotic bacteria on gut microbiota composition and gut wall function in an in-vitro model in patients with Parkinson's disease

GHYSELINCK, Jonas, VERSTREPEN, Lynn, MOENS, Frédéric, VAN DEN ABBEELE, Pieter, BRUGGEMAN, Arnout, SAID, Jawal, SMITH, Barry, BARKER, Lynne, JORDAN, Caroline, LETA, Valentina, CHAUDHURI, K. Ray, BASIT, Abdul W. and GAISFORD, Simon (2021). Influence of probiotic bacteria on gut microbiota composition and gut wall function in an in-vitro model in patients with Parkinson's disease. International Journal of Pharmaceutics: X, 3, p. 100087.

[img]
Preview
PDF
Barker-InfluenceProbioticBacteria(VoR).pdf - Published Version
Creative Commons Attribution Non-commercial No Derivatives.

Download (2MB) | Preview
Official URL: https://www.sciencedirect.com/science/article/pii/...
Open Access URL: https://www.sciencedirect.com/science/article/pii/... (Published version)
Link to published version:: https://doi.org/10.1016/j.ijpx.2021.100087
Related URLs:

    Abstract

    We report here the potential role of a 4-strain probiotic suspension for use with patients with Parkinson's disease (PD). Stool samples from a group of three patients with diagnosed PD were used to create microbiotas in an in-vitro gut model. The effects of dosing with an oral probiotic suspension (Symprove) on bacterial composition and metabolic activity in the microbiotas was evaluated over 48 h and compared with healthy controls. Additionally, the effect of probiotic dosing on epithelial tight-junction integrity, production of inflammatory markers and wound healing were evaluated in cell culture models. In general, the relative proportions of the main bacterial phyla in the microbiotas of PD patients differed from those of healthy subjects, with levels of Firmicutes raised and levels of Bacteroidetes reduced. Dosing with probiotic resulted in a change in bacterial composition in the microbiotas over a 48 h period. Several other indicators of gut health changed upon dosing with the probiotic; production of short chain fatty acids (SCFAs) and lactate was stimulated, levels of anti-inflammatory cytokines (IL-6, IL-10) increased and levels of pro-inflammatory cytokines and chemokines (MCP-1 and IL-8) decreased. Tight junction integrity was seen to improve with probiotic dosing and wound healing was seen to occur faster than a control. The data suggest that if development and/or progression of PD is influenced by gut microbiota dysbiosis then supplementation of the diet with a properly formulated probiotic may be a useful adjunct to standard treatment in clinic.

    Item Type: Article
    Identification Number: https://doi.org/10.1016/j.ijpx.2021.100087
    Page Range: p. 100087
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 19 Jul 2021 09:27
    Last Modified: 02 Aug 2021 08:32
    URI: http://shura.shu.ac.uk/id/eprint/28847

    Actions (login required)

    View Item View Item

    Downloads

    Downloads per month over past year

    View more statistics