Antigen Retrieval and Its Effect on the MALDI-MSI of Lipids in Formalin-Fixed Paraffin-Embedded Tissue

DENTI, V., PIGA, I., GUARNERIO, Sonia, CLERICI, F., IVANOVA, M., CHINELLO, C., PAGLIA, G., MAGNI, F. and SMITH, A. (2020). Antigen Retrieval and Its Effect on the MALDI-MSI of Lipids in Formalin-Fixed Paraffin-Embedded Tissue. Journal of the American Society for Mass Spectrometry, 31 (8), 1619-1624.

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Open Access URL: https://pubs.acs.org/doi/10.1021/jasms.0c00208 (Published version)
Link to published version:: https://doi.org/10.1021/jasms.0c00208
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    Abstract

    Formalin-fixed paraffin-embedded (FFPE) tissue represents the primary source of clinical tissue and is routinely used in MALDI-MSI studies. However, it is not particularly suitable for lipidomics imaging given that many species are depleted during tissue processing. Irrespective, a number of solvent-resistant lipids remain, but their extraction may be hindered by the cross-link between proteins. Therefore, an antigen retrieval step could enable the extraction of a greater number of lipids and may provide information that is complementary to that which can be obtained from other biomolecules, such as proteins. In this short communication, we aim to address the effect of performing antigen retrieval prior to MALDI-MSI of lipids in FFPE tissue. As a result, an increased number of lipid signals could be detected and may have derived from lipid species that are known to be implicated in the lipid-protein cross-linking that is formed as a result of formalin fixation. Human renal cancer tissue was used as a proof of concept to determine whether using these detected lipid signals were also able to highlight the histopathological regions that were present. These preliminary findings may highlight the potential to enhance the clinical relevance of the lipidomic information obtained from FFPE tissue.

    Item Type: Article
    Uncontrolled Keywords: Lipids; Lipidomics; FFPE tissue; MALDI-MS; Imaging mass spectrometry; FFPE tissue; Imaging mass spectrometry; Lipidomics; Lipids; MALDI-MS; Analytical Chemistry; 0301 Analytical Chemistry; 0304 Medicinal and Biomolecular Chemistry; 0306 Physical Chemistry (incl. Structural)
    Identification Number: https://doi.org/10.1021/jasms.0c00208
    Page Range: 1619-1624
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 26 Apr 2021 10:04
    Last Modified: 26 Apr 2021 10:15
    URI: http://shura.shu.ac.uk/id/eprint/28551

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