Electrochemical Detection of Prostate Cancer Biomarker PCA3 Using Specific RNA-Based Aptamer Labelled with Ferrocene

NABOK, Aleksey, ABU-ALI, Hisham, TAKITA, Sarra and SMITH, David P. (2021). Electrochemical Detection of Prostate Cancer Biomarker PCA3 Using Specific RNA-Based Aptamer Labelled with Ferrocene. Chemosensors, 9 (4).

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Open Access URL: https://www.mdpi.com/2227-9040/9/4/59 (Published version)
Link to published version:: https://doi.org/10.3390/chemosensors9040059
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    Abstract

    This paper reports on a feasibility study of electrochemical in-vitro detection of prostate cancer biomarker PCA3 (prostate cancer antigen 3) in direct assay with specific RNA aptamer labelled with a redox group (ferrocene) and immobilized on a screen-printed gold electrode surface. The cyclic voltammograms and electrochemical impedance spectroscopy methods yield encouraging results on the detection of PCA3 in a range of concentrations from 1 μg/mL down to 0.1 ng/mL in buffer solutions. Both anodic and cathodic current values in cyclic voltammograms measurements and charge transfer resistance values in electrochemical impedance spectroscopy experiments correlate with the PCA3 concentration in the sample. Kinetics studies of the binding of the PCA3 to our aptamer demonstrated high specificity of the reaction with a characteristic affinity constant of approximately 4·10−10 molar. The results of this work provide a background for the future development of novel, highly sensitive and cost-effective diagnostic methodologies for prostate cancer detection.

    Item Type: Article
    Additional Information: ** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2227-9040 **History: published 24-03-2021; accepted 21-03-2021
    Uncontrolled Keywords: prostate cancer, RNA transcript PCA3, aptamer, electrochemical biosensor, cyclic voltammograms, impedance spectroscopy
    Identification Number: https://doi.org/10.3390/chemosensors9040059
    SWORD Depositor: Colin Knott
    Depositing User: Colin Knott
    Date Deposited: 30 Mar 2021 11:26
    Last Modified: 30 Mar 2021 11:26
    URI: http://shura.shu.ac.uk/id/eprint/28456

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