Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging.

ANGELINI, Roberto, YUTUC, Eylan, WYATT, Mark F., NEWTON, Jillian, YUSUF, Fowzi A., GRIFFITHS, Lauren, COOZE, Benjamin J., EL ASSAD, Dana, FRACHE, Gilles, RAO, Wei, ALLEN, Luke B., KORADE, Zeljka, NGUYEN, Thu T.A., RATHNAYAKE, Rathnayake A.C., COLOGNA, Stephanie M., HOWELL, Owain W., CLENCH, Malcolm R, WANG, Yuqin and GRIFFITHS, William J (2021). Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging. Analytical Chemistry.

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Open Access URL: https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.... (Published version)
Link to published version:: https://doi.org/10.1021/acs.analchem.0c05399
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    Abstract

    Despite being a critical molecule in the brain, mass spectrometry imaging (MSI) of cholesterol has been under-reported compared to other lipids due to the difficulty in ionizing the sterol molecule. In the present work, we have employed an on-tissue enzyme-assisted derivatization strategy to improve detection of cholesterol in brain tissue sections. We report distribution and levels of cholesterol across specific structures of the mouse brain, in a model of Niemann-Pick type C1 disease, and during brain development. MSI revealed that in the adult mouse, cholesterol is the highest in the pons and medulla and how its distribution changes during development. Cholesterol was significantly reduced in the corpus callosum and other brain regions in the Npc1 null mouse, confirming hypomyelination at the molecular level. Our study demonstrates the potential of MSI to the study of sterols in neuroscience.

    Item Type: Article
    Uncontrolled Keywords: Analytical Chemistry; 0301 Analytical Chemistry; 0399 Other Chemical Sciences
    Identification Number: https://doi.org/10.1021/acs.analchem.0c05399
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 19 Mar 2021 11:49
    Last Modified: 19 Mar 2021 12:01
    URI: http://shura.shu.ac.uk/id/eprint/28419

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