Application of Comprehensive 2D Chromatography in the Anti-Doping Field: Sample Identification and Quantification

HAND, Rachel A, BASSINDALE, Thomas, TURNER, Nicholas and MORGAN, Geraint (2021). Application of Comprehensive 2D Chromatography in the Anti-Doping Field: Sample Identification and Quantification. Journal of Chromatography B, p. 122584.

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Link to published version:: https://doi.org/10.1016/j.jchromb.2021.122584
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    Abstract

    Anti-doping analysis requires an exceptional level of accuracy and precision given the stakes that are at play. Current methods rely on the application of chromatographic techniques linked with mass spectrometry to provide this. However, despite the effectiveness of these techniques in achieving good selectivity and specificity, some issues still exist. In order to reach the minimum required performance level as set by WADA, labs commonly use selective monitoring by quadrupole mass spectrometry. This can be potentially fooled through the use of masking agents or by moving the peaks, as often only a small portion of the spectrum is used for analysis. Further issues exist in the inability to detect new or modified compounds, or to reanalyse samples/spectra. One technique that could overcome these problems is that of comprehensive 2D chromatography. Here a second separation column is employed to generate greater separative power. Compared to conventional separation, GCxGC allows for a greater peak capacity (i.e., number of peaks that can be resolved within a given time) and greater separation of coeluting compounds, which makes the technique promising for the complex task required in anti-doping. When combined with Time of Flight Mass Spectrometry this technique demonstrates vast potential allowing for full mass range datasets to be obtained for retroactive analysis. Similarly, LCxLC provides improvements in resolving power compared to its 1D counterpart and can be used both online as part of the analysis or offline solely as a purification step. In this review we summarise the work in this field so far, how comprehensive chromatography has been applied to anti-doping studies, and discuss the future application for this technique.

    Item Type: Article
    Uncontrolled Keywords: 0301 Analytical Chemistry; 0601 Biochemistry and Cell Biology; 1115 Pharmacology and Pharmaceutical Sciences
    Identification Number: https://doi.org/10.1016/j.jchromb.2021.122584
    Page Range: p. 122584
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 23 Feb 2021 16:49
    Last Modified: 17 Mar 2021 14:45
    URI: http://shura.shu.ac.uk/id/eprint/28201

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