Osmotic adaptation of nucleus pulposus cells: The role of aquaporin 1, aquaporin 4 and transient receptor potential vanilloid 4

SNUGGS, J.W., BUNNING, R. and LE MAITRE, Christine Lyn (2021). Osmotic adaptation of nucleus pulposus cells: The role of aquaporin 1, aquaporin 4 and transient receptor potential vanilloid 4. eCells and Materials Journal, 41, 121-141.

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    Abstract

    The microenvironment of the nucleus pulposus is hyperosmotic and fluctuates diurnally due to mechanical loading. Changes in extracellular osmolality result in cell volume alterations, responsiveness to such changes is essential for cellular homeostasis. Aquaporins allow movement of water across cell membranes and control water permeability in response to osmotic gradients. Furthermore, transient receptor potential vanilloid 4 has been shown to sense osmotic and mechanical stimuli resulting in changes to intracellular Ca2+. It has been shown previously that aquaporin 1 and 4 expression decreases during disc degeneration. Here, the expression of transient receptor potential vanilloid 4 by human nucleus pulposus cells during disc degeneration, and the roles of aquaporin 1, 4 and transient receptor potential vanilloid 4 in regulating responses to osmotic gradients was investigated. Transient receptor potential vanilloid 4 was expressed by the majority of human nucleus pulposus cells and not affected by disc degeneration. Aquaporin 4 staining co-localised with primary cilia. Nucleus pulposus cells modulated their rate of volume change, water permeability and Ca2+ influx in response to extracellular osmolality. These responses were inhibited by chemical inhibition of aquaporin 4, transient receptor potential vanilloid 4, and to a lesser extent aquaporin 1; suggesting that both aquaporins and transient receptor potential vanilloid 4 play important roles in the fundamental adaptation of nucleus pulposus cells to their osmotic environment. Co-localisation with primary cilia indicates these proteins may function synergistically to achieve adaptation, which may be lost during disc degeneration, when aquaporin 1 and 4 expression is reduced.

    Item Type: Article
    Additional Information: With kind permission of full reproduction from eCM journal (www.ecmjournal.org). Founded by scientists for the benefit of science rather than profit.
    Uncontrolled Keywords: Biomedical Engineering; 0601 Biochemistry and Cell Biology; 0903 Biomedical Engineering
    Identification Number: https://doi.org/10.22203/eCM
    Page Range: 121-141
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 14 Jan 2021 16:14
    Last Modified: 17 Mar 2021 15:15
    URI: http://shura.shu.ac.uk/id/eprint/27980

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