In Vivo Water Dynamics in Shewanella oneidensis Bacteria at High Pressure

FOGLIA, Fabrizia, HAZAEL, Rachael, MEERSMAN, Filip, WILDING, Martin, SAKAI, Victoria García, ROGERS, Sarah, BOVE, Livia E., KOZA, Michael Marek, MOULIN, Martine, HAERTLEIN, Michael, FORSYTH, V. Trevor and MCMILLAN, Paul F. (2019). In Vivo Water Dynamics in Shewanella oneidensis Bacteria at High Pressure. Scientific Reports, 9 (1), p. 8716.

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Open Access URL: https://www.nature.com/articles/s41598-019-44704-3 (Published version)
Link to published version:: https://doi.org/10.1038/s41598-019-44704-3
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    Abstract

    Abstract: Following observations of survival of microbes and other life forms in deep subsurface environments it is necessary to understand their biological functioning under high pressure conditions. Key aspects of biochemical reactions and transport processes within cells are determined by the intracellular water dynamics. We studied water diffusion and rotational relaxation in live Shewanella oneidensis bacteria at pressures up to 500 MPa using quasi-elastic neutron scattering (QENS). The intracellular diffusion exhibits a significantly greater slowdown (by −10–30%) and an increase in rotational relaxation times (+10–40%) compared with water dynamics in the aqueous solutions used to resuspend the bacterial samples. Those results indicate both a pressure-induced viscosity increase and slowdown in ionic/macromolecular transport properties within the cells affecting the rates of metabolic and other biological processes. Our new data support emerging models for intracellular organisation with nanoscale water channels threading between macromolecular regions within a dynamically organized structure rather than a homogenous gel-like cytoplasm.

    Item Type: Article
    Additional Information: ** From Springer Nature via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2045-2322 **Article IDs: publisher-id: s41598-019-44704-3; manuscript: 44704 **History: collection 12-2019; published_online 18-06-2019; online 18-06-2019; registration 23-05-2019; accepted 15-05-2019; submitted 18-02-2019
    Uncontrolled Keywords: Article, /631/57/2268, /639/766/747, /128, article
    Identification Number: https://doi.org/10.1038/s41598-019-44704-3
    Page Range: p. 8716
    SWORD Depositor: Colin Knott
    Depositing User: Colin Knott
    Date Deposited: 17 Jun 2020 16:14
    Last Modified: 17 Jun 2020 16:15
    URI: http://shura.shu.ac.uk/id/eprint/26478

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