Electrochemical Aptasensor for Detection of Dopamine

ABU-ALI, Hisham, OZKAYA, Cansu, DAVIS, Frank, WALCH, Nik and NABOK, Alexei (2020). Electrochemical Aptasensor for Detection of Dopamine. Chemosensors, 8 (2), e28.

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Abstract

This work presents a proof of concept of a novel, simple, and sensitive method of detection of dopamine, a neurotransmitter within the human brain. We propose a simple electrochemical method for the detection of dopamine using a dopamine-specific aptamer labeled with an electrochemically active ferrocene tag. Aptamers immobilized on the surface of gold screen-printed gold electrodes via thiol groups can change their secondary structure by wrapping around the target molecule. As a result, the ferrocene labels move closer to the electrode surface and subsequently increase the electron transfer. The cyclic voltammograms and impedance spectra recorded on electrodes in buffer solutions containing different concentration of dopamine showed, respectively, the increase in both the anodic and cathodic currents and decrease in the double layer resistance upon increasing the concentration of dopamine from 0.1 to 10 nM L-1. The high affinity of aptamer-dopamine binding (KD ≈ 5 nM) was found by the analysis of the binding kinetics. The occurrence of aptamer-dopamine binding was directly confirmed with spectroscopic ellipsometry measurements.

Item Type:	Article
Additional Information:	** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2227-9040 **History: published 15-04-2020; accepted 11-04-2020
Uncontrolled Keywords:	dopamine, electrochemical biosensor, aptamer, cyclic voltammetry, electrochemical impedance spectroscopy, spectroscopic ellipsometry
Identification Number:	https://doi.org/10.3390/chemosensors8020028
Page Range:	e28
SWORD Depositor:	Colin Knott
Depositing User:	Colin Knott
Date Deposited:	19 Apr 2020 16:28
Last Modified:	18 Mar 2021 01:17
URI:	https://shura.shu.ac.uk/id/eprint/26133

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