Lipid changes within the epidermis of living skin equivalents observed across a time-course by MALDI-MS imaging and profiling

MITCHELL, C.A., LONG, H., DONALDSON, M., FRANCESE, S. and CLENCH, Malcolm (2015). Lipid changes within the epidermis of living skin equivalents observed across a time-course by MALDI-MS imaging and profiling. Lipids in Health and Disease, 14 (1), p. 84.

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Official URL: https://lipidworld.biomedcentral.com/articles/10.1...
Link to published version:: https://doi.org/10.1186/s12944-015-0089-z
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    Abstract

    © 2015 Mitchell et al. Abstract Background: Mass spectrometry imaging (MSI) is a powerful tool for the study of intact tissue sections. Here, its application to the study of the distribution of lipids in sections of reconstructed living skin equivalents during their development and maturation is described. Methods: Living skin equivalent (LSE) samples were obtained at 14 days development, re-suspended in maintenance medium and incubated for 24 h after delivery. The medium was then changed, the LSE re-incubated and samples taken at 4, 6 and 24 h time points. Mass spectra and mass spectral images were recorded from 12 μm sections of the LSE taken at each time point for comparison using matrix assisted laser desorption ionisation mass spectrometry. Results: A large number of lipid species were identified in the LSE via accurate mass-measurement MS and MSMS experiments carried out directly on the tissue sections. MS images acquired at a spatial resolution of 50 μm × 50 μm showed the distribution of identified lipids within the developing LSE and changes in their distribution with time. In particular development of an epidermal layer was observable as a compaction of the distribution of phosphatidylcholine species. Conclusions: MSI can be used to study changes in lipid composition in LSE. Determination of the changes in lipid distribution during the maturation of the LSE will assist in the identification of treatment responses in future investigations.

    Item Type: Article
    Uncontrolled Keywords: MALDI-MSI; MALDI; Mass spectrometry imaging; MSI; Lipids; Living skin equivalents; Epidermis; Humans; Imaging, Three-Dimensional; Lipids; Multivariate Analysis; Principal Component Analysis; Skin, Artificial; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sphingomyelins; Tandem Mass Spectrometry; Time Factors; Epidermis; Humans; Sphingomyelins; Lipids; Imaging, Three-Dimensional; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Multivariate Analysis; Skin, Artificial; Principal Component Analysis; Time Factors; Tandem Mass Spectrometry; Nutrition & Dietetics; 1101 Medical Biochemistry and Metabolomics; 0899 Other Information and Computing Sciences
    Identification Number: https://doi.org/10.1186/s12944-015-0089-z
    Page Range: p. 84
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 16 Apr 2020 16:38
    Last Modified: 16 Apr 2020 16:45
    URI: http://shura.shu.ac.uk/id/eprint/25951

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