Cytotoxic effects of Smp24 and Smp43 scorpion venom antimicrobial peptides on tumour and non-tumour cell lines

ELRAYESS, Ranwa A., MOHALLAL, Mahmoud E, EL-SHAHAT, Yomn M, EBAID, Hala M, MILLER, Keith, STRONG, Peter and ABDEL-RAHMAN, Mohamed A (2019). Cytotoxic effects of Smp24 and Smp43 scorpion venom antimicrobial peptides on tumour and non-tumour cell lines. International Journal of Peptide Research and Therapeutics.

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Official URL: https://link.springer.com/article/10.1007/s10989-0...
Open Access URL: https://link.springer.com/content/pdf/10.1007%2Fs1... (Published)
Link to published version:: https://doi.org/10.1007/s10989-019-09932-1

Abstract

Smp24 and Smp43 are novel cationic AMPs identified from the venom of the Egyptian scorpion Scorpio maurus palmatus, having potent activity against both Gram-positive and Gram-negative bacteria as well as fungi. Here we describe cytotoxicity of these peptides towards three non-tumour cell lines (CD34+ (hematopoietic stem progenitor from cord blood), HRECs (human renal epithelial cells) and HACAT (human skin keratinocytes) and two acute leukaemia cell lines (myeloid (KG1a) and lymphoid (CCRF-CEM) leukaemia cell lines) using a combination of biochemical and imaging techniques. Smp24 and Smp43 (4–256 µg/mL) decreased the cell viability (as measured by intracellular ATP) of all cells tested, although keratinocytes were markedly less sensitive. Cell membrane leakage as evidenced by the release of lactate dehydrogenase was evident throughout and was confirmed by scanning electron microscope studies.

Item Type: Article
Uncontrolled Keywords: 0601 Biochemistry and Cell Biology; 1101 Medical Biochemistry and Metabolomics; Organic Chemistry
Identification Number: https://doi.org/10.1007/s10989-019-09932-1
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 29 Oct 2019 16:29
Last Modified: 29 Oct 2019 16:30
URI: http://shura.shu.ac.uk/id/eprint/25299

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