Development of a novel electrochemical inhibition sensor array based on bacteria immobilized on modified screen-printed gold electrodes for water pollution detection

ABU-ALI, H, NABOK, Aleksey, SMITH, Thomas and AL-SHANAWA, M (2019). Development of a novel electrochemical inhibition sensor array based on bacteria immobilized on modified screen-printed gold electrodes for water pollution detection. BioNanoScience, 9 (2), 345-355.

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Official URL: https://link.springer.com/article/10.1007%2Fs12668...
Open Access URL: https://link.springer.com/content/pdf/10.1007%2Fs1... (Published)
Link to published version:: https://doi.org/10.1007/s12668-019-00619-x

Abstract

The development of a novel and simple inhibition biosensor array for detection of water pollutants based on bacteria immobilized on the surface of the electrodes is the main goal of this work. A series of electrochemical measurements (i.e., cyclic voltammograms) were carried out on modified screen-printed gold electrodes with three types of bacteria, namely Escherichia coli, Shewanella oneidensis, and Methylococcus capsulatus (Bath), immobilized via poly l-lysine. For comparison purposes, similar measurements were carried out on bacteria samples in solutions; also optical measurements (fluorescence microscopy, optical density, and flow cytometry) were performed on the same bacteria in both liquid and immobilized forms. The study of the effect of heavy metal ions (lead), pesticides (atrazine), and petrochemicals (hexane) on DC electrochemical characteristics of immobilized bacteria revealed a possibility of pattern recognition of the above inhibition agents in an aquatic environment.

Item Type: Article
Uncontrolled Keywords: Electrochemical sensor; Inhibition bacteria sensor array; Immobilization of bacteria; Water pollution; Pattern recognition
Identification Number: https://doi.org/10.1007/s12668-019-00619-x
Page Range: 345-355
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 10 Jun 2019 14:07
Last Modified: 10 Jun 2019 14:07
URI: http://shura.shu.ac.uk/id/eprint/24677

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