MicroRNA-155 is essential for the optimal proliferation and survival of plasmablast B cells.

ARBORE, Giuseppina, HENLEY, Tom, BIGGINS, Laura, ANDREWS, Simon, VIGORITO, Elena, TURNER, Martin and LEYLAND, Rebecca (2019). MicroRNA-155 is essential for the optimal proliferation and survival of plasmablast B cells. Life Science Alliance, 2 (3), e201800244.

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    Abstract

    A fast antibody response can be critical to contain rapidly dividing pathogens. This can be achieved by the expansion of antigen-specific B cells in response to T-cell help followed by differentiation into plasmablasts. MicroRNA-155 (miR-155) is required for optimal T-cell-dependent extrafollicular responses via regulation of PU.1, although the cellular processes underlying this defect are largely unknown. Here, we show that miR-155 regulates the early expansion of B-blasts and later on the survival and proliferation of plasmablasts in a B-cell-intrinsic manner, by tracking antigen-specific B cells in vivo since the onset of antigen stimulation. In agreement, comparative analysis of the transcriptome of miR-155-sufficient and miR-155-deficient plasmablasts at the peak of the response showed that the main processes regulated by miR-155 were DNA metabolic process, DNA replication, and cell cycle. Thus, miR-155 controls the extent of the extrafollicular response by regulating the survival and proliferation of B-blasts, plasmablasts and, consequently, antibody production.

    Item Type: Article
    Identification Number: https://doi.org/10.26508/lsa.201800244
    Page Range: e201800244
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 05 Jun 2019 15:29
    Last Modified: 05 Jun 2019 15:30
    URI: http://shura.shu.ac.uk/id/eprint/24652

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