Flow cytometric phenotyping of diverse human cancer cell lines for immunological biomarkers expression

HUDSON, Katie, CROSS, Neil, JORDAN-MAHY, Nicola and LEYLAND, Rebecca (2018). Flow cytometric phenotyping of diverse human cancer cell lines for immunological biomarkers expression. In: BMRC/MERI Winter Poster Event, Sheffield, 14 Dec 2018. BMRC/MERI. (Unpublished)

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    Abstract

    The tumour microenvironment contains a variety of distinct factors that inhibit the immune system and can cause drug resistance. Some of these factors include the expression of cell surface markers which interact directly with immune cells. Cancer cells express programmed death ligand 1 (PD-L1) and reduce the expression of major-histocompatibility complex class I, death-receptors 4/5 and Fas, limiting immune-mediated cancer cell killing. Targeting these immune markers alone or in combination could potentially increase cancer cell death and improve drug efficacy. Utilising flow cytometric analysis on breast, prostate and colorectal cancer cell lines, we have found differential expression of these markers depending on the cancer type. These findings provide a platform for future work that will entail siRNA knockdown of PD-L1 to determine the tumour-intrinsic role of this ligand, in addition to combination therapies in 2D and 3D cell culture.

    Item Type: Conference or Workshop Item (Poster)
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 11 Feb 2019 13:55
    Last Modified: 11 Feb 2019 14:00
    URI: http://shura.shu.ac.uk/id/eprint/23638

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