Quantitative investigation of terbinafine hydrochloride absorption into a living skin equivalent model by MALDI-MSI

RUSSO, Cristina, BRICKELBANK, Neil, DUCKETT, Catherine, MELLOR, Steve, RUMBELOW, Stephen and CLENCH, Malcolm (2018). Quantitative investigation of terbinafine hydrochloride absorption into a living skin equivalent model by MALDI-MSI. Analytical chemistry, 90 (16), 10031-10038.

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Official URL: https://pubs.acs.org/doi/10.1021/acs.analchem.8b02...
Link to published version:: https://doi.org/10.1021/acs.analchem.8b02648


The combination of microspotting of analytical and internal standards, matrix sublimation, and recently developed software for quantitative mass spectrometry imaging has been used to develop a high-resolution method for the determination of terbinafine hydrochloride in the epidermal region of a full thickness living skin equivalent model. A quantitative assessment of the effect of the addition of the penetration enhancer (dimethyl isosorbide (DMI)) to the delivery vehicle has also been performed, and data have been compared to those obtained from LC-MS/MS measurements of homogenates of isolated epidermal tissue. At 10% DMI, the levels of signal detected for the drug in the epidermis were 0.20 ± 0.072 mg/g tissue for QMSI and 0.28 ± 0.040 mg/g tissue for LC-MS/MS at 50% DMI 0.69 ± 0.23 mg/g tissue for QMSI and 0.66 ± 0.057 mg/g tissue for LC-MS/MS. Comparison of means and standard deviations indicates no significant difference between the values obtained by the two methods.

Item Type: Article
Additional Information: ** From PubMed via Jisc Publications Router.
Research Institute, Centre or Group - Does NOT include content added after October 2018: Biomedical Research Centre
Identification Number: https://doi.org/10.1021/acs.analchem.8b02648
Page Range: 10031-10038
SWORD Depositor: Margaret Boot
Depositing User: Margaret Boot
Date Deposited: 09 Aug 2018 13:56
Last Modified: 18 Mar 2021 01:15
URI: https://shura.shu.ac.uk/id/eprint/22212

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