Label-free optical detection of mycotoxins using specific aptamers immobilized on gold nanostructures.

GHANIM AL-RUBAYE, Ali, NABOK, Aleksey, CATANANTE, Gaelle, MARTY, Jean-Louis, TAKÁCS, Eszter and SZÉKÁCS, András (2018). Label-free optical detection of mycotoxins using specific aptamers immobilized on gold nanostructures. Toxins, 10 (7), p. 291.

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Official URL: http://www.mdpi.com/2072-6651/10/7/291
Link to published version:: https://doi.org/10.3390/toxins10070291
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    Abstract

    This work focuses on the development of the novel label-free optical apta-sensors for detection of mycotoxins. A highly sensitive analytical method of total internal reflection ellipsometry (TIRE) combined with Localized Surface Plasmon Resonance (LSPR) phenomenon in nano-structured gold films was exploited here for the first time for detection of aflatoxin B1 and M1 in direct assay with specific aptamers immobilized on the surface of gold. The achieved detection of low molecular weight molecules, such as aflatoxin B1 and M1, in a wide range of concentrations from 100 ng/mL down to 0.01 ng/mL is remarkable for the LSPR method. The study of binding kinetics of aflatoxin molecules to their respective aptamers using dynamic TIRE measurements yielded the values of affinity constants in the range of 10 ⁻10 mol, which is characteristic for highly specific aptamer/target interactions similar to that for monoclonal antibodies. The effect of aptamers' DNA chain length on their binding characteristics was analyzed.

    Item Type: Article
    Additional Information: ** From PubMed via Jisc Publications Router. ** History: received 26-06-2018; revised 12-07-2018; accepted 13-07-2018.
    Uncontrolled Keywords: LSPR, aptamer, gold nanostructures, mycotoxins, optical label-free biosensor, total internal reflection ellipsometery
    Research Institute, Centre or Group - Does NOT include content added after October 2018: Materials and Engineering Research Institute > Thin Films Research Centre > Electronic Materials and Sensors Research Group
    Identification Number: https://doi.org/10.3390/toxins10070291
    Page Range: p. 291
    SWORD Depositor: Margaret Boot
    Depositing User: Margaret Boot
    Date Deposited: 08 Aug 2018 10:23
    Last Modified: 16 Nov 2018 14:12
    URI: http://shura.shu.ac.uk/id/eprint/22169

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