Determination of free 25(OH)D concentrations and their relationships to total 25(OH)D in multiple clinical populations

SCHWARTZ, JB, GALLAGHER, C, JORDE, R, BERG, V, WALSH, J, EASTELL, R, EVANS, AL, BOWLES, Simon, NAYLOR, KE, JONES, KS, SCHOENMAKERS, I, HOLICK, M, ORWELL, E, NIELSON, C, KAUFMAN, M, JONES, G, BOUILLON, R, LAI, J, VEROTTA, D and BIKLE, D (2018). Determination of free 25(OH)D concentrations and their relationships to total 25(OH)D in multiple clinical populations. Journal of Clinical Endocrinology & Metabolism.

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Link to published version:: https://doi.org/10.1210/jc.2018-00295
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    Abstract

    Context : The optimal measure of vitamin D(D) status is unknown. Objective : Directly measure circulating free 25(OH)D concentrations and relationships to total 25(OH)D in a clinically diverse sample of humans. Design : Cross-sectional analysis Setting : Seven academic sites Patients : 1661 adults: (healthy(n=211), pre-diabetic(n=479), outpatients(n=783), cirrhotic(n=90), pregnant(n=20), nursing home(n=79)) Interventions : Merge research data on circulating free 25(OH)D (directly measured immunoassay), total 25(OH)D (LC/MS/MS), D binding protein (DBP by radial (polyclonal) immunodiffusion assay)), albumin, creatinine, iPTH and DBP haplotype Main outcome measures : Distribution of free 25(OH)D (ANOVA with Bonferroni correction for post hoc comparisons) and relationships between free and total 25(OH)D (mixed effects modeling incorporating clinical condition, DBP haplotype with sex, race, eGFR, BMI and other covariates). Results : Free 25(OH)D was 4.7±1.8 pg/mL (mean ±SD) in healthy and 4.3 ±1.9 pg/mL in outpatients with 0.5-8.1 pg/mL and 0.9-8.1 pg/mL encompassing 95% of healthy and outpatients, respectively. Free 25(OH)D was higher in cirrhotics (7.1 ±3.0 pg/mL, p<.0033) and nursing home residents (7.9± 2.1pg/mL, p<.0033) compared to other groups and differed between whites and blacks (p<.0033) and between DBP haplotypes (p<.0001). Mixed effects modeling of relationships between free and total 25(OH)D identified clinical conditions (cirrhotic>nursing home>prediabetic > outpatient > pregnant), and BMI (lesser effect) as covariates affecting relationships but not eGFR, sex, race or DBP haplotype. Conclusions : Total 25(OH)D, health condition, race and DBP haplotype affected free 25(OH)D, but only health conditions and BMI affected relationships between total and free 25(OH) D. Clinical importance of free 25(OH)D needs to be established in studies assessing outcomes.

    Item Type: Article
    Research Institute, Centre or Group - Does NOT include content added after October 2018: Sheffield Business School Research Institute > Service Sector Management
    Departments - Does NOT include content added after October 2018: Sheffield Business School > Department of Service Sector Management
    Identification Number: https://doi.org/10.1210/jc.2018-00295
    Depositing User: Simon Bowles
    Date Deposited: 30 Jul 2018 14:01
    Last Modified: 27 Jun 2019 01:18
    URI: http://shura.shu.ac.uk/id/eprint/22149

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