Next-generation sequencing showing potential leachate influence on bacterial communities around a landfill in China

RAJASEKAR, Adharsh, RAJU, Sekar, MEDINA-ROLDAN, Eduardo, BRIDGE, Jonathan, MOY, Charles K.S and WILKINSON, Stephen (2018). Next-generation sequencing showing potential leachate influence on bacterial communities around a landfill in China. Canadian Journal of Microbiology. (In Press)

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Official URL: http://www.nrcresearchpress.com/doi/10.1139/cjm-20...
Link to published version:: 10.1139/cjm-2017-0543

Abstract

The impact of contaminated leachate on groundwater from landfills is well known but specific effects on bacterial consortia are less well-studied. Bacterial communities in landfill and an urban site located in Suzhou, China were studied using Illumina high-throughput sequencing. A total number of 153944 good quality reads were produced and sequences assigned to 6388 operational taxonomic units (OTUs). Bacterial consortia consisted of up to 16 phyla including Proteobacteria (31.9 to 94.9% at landfill, 25.1 to 43.3% at urban sites), Actinobacteria (0 to 28.7% at landfill, 9.9 to 34.3% at urban sites), Bacteroidetes (1.4 to 25.6% at landfill, 5.6 to 7.8% at urban sites), Chloroflexi (0.4 to 26.5% at urban sites only) and unclassified bacteria. Pseudomonas was the dominant (67-93%) genus in landfill leachate. Arsenic concentrations in landfill raw leachate (RL) (1.11x103 µg/L) and fresh leachate (FL2) (1.78x103 µg/L), and mercury concentrations in RL (10.9 µg/L) and FL2 (7.37 µg/L) were higher than Chinese State Environmental Protection Administration (SEPA) standards for leachate in landfills. Shannon diversity index and Chao 1 richness estimate showed RL and FL2 lacked richness and diversity when compared with other samples. This is consistent with stresses imposed by elevated arsenic and mercury and has implications for ecological site remediation by bioremediation or natural attenuation.

Item Type: Article
Additional Information: ** From Crossref via Jisc Publications Router.
Uncontrolled Keywords: Immunology, Applied Microbiology and Biotechnology, Genetics, Molecular Biology, Microbiology, General Medicine
Identification Number: 10.1139/cjm-2017-0543
SWORD Depositor: Margaret Boot
Depositing User: Margaret Boot
Date Deposited: 16 Apr 2018 11:14
Last Modified: 16 Apr 2018 11:14
URI: http://shura.shu.ac.uk/id/eprint/20871

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