MALDI-MS imaging for direct drug distribution analysis.

TRIM, Paul James. (2009). MALDI-MS imaging for direct drug distribution analysis. Doctoral, Sheffield Hallam University (United Kingdom)..

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    Abstract

    MALDI Imaging has gained huge interest in the past few years with an ever increasing population of specialists choosing to investigate samples using MALDI imaging, including growing interest and financial backing from pharma and contract research organisations.Presented within this thesis is the development and application of MALDI imaging techniques for a variety of analytical problems. The use of various software packages have been employed in the interpretation of the data acquired from MALDI experiments including, the use of statistical analysis for the identification of ion of interest from 6 distinct brain regions and also for the identification of ions of interest associated with small molecule tumour markers.The advantages of MALDI-IMS-MSI as a further separation stage within MALDI-MSI have been shown. Demonstrated is a method for MALDI-IMS-MS imaging of endogenous lipids in healthy tissue and tumours, also demonstrated is the application of MALDI-IMS-MS to xenobiotic distribution studies, it has been clearly shown that ion mobility separation within MALDI-MSI experiments can improve the analysis of xenobiotics by removing any interfering ions.With instrumentation development for MALDI a high repetition rate Nd:YVO4 laser has been assessed as a possible method for decreasing acquisition time.

    Item Type: Thesis (Doctoral)
    Additional Information: Thesis (Ph.D.)--Sheffield Hallam University (United Kingdom), 2009.
    Research Institute, Centre or Group - Does NOT include content added after October 2018: Sheffield Hallam Doctoral Theses
    Depositing User: EPrints Services
    Date Deposited: 10 Apr 2018 17:22
    Last Modified: 26 Apr 2021 12:33
    URI: http://shura.shu.ac.uk/id/eprint/20455

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