Real-time polymerase chain reaction shows that density centrifugation does not always remove Chlamydia trachomatis from human semen

AL-MOUSLY, N., CROSS, N. A., ELEY, A. and PACEY, A. A. (2009). Real-time polymerase chain reaction shows that density centrifugation does not always remove Chlamydia trachomatis from human semen. Fertility and sterility, 92 (5), 1606-1615.

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Link to published version:: 10.1016/j.fertnstert.2008.08.128

Abstract

Objective: To evaluate the efficiency of sperm washing procedures to remove Chlamydia trachomatis from semen both in clinical samples and experimental inoculations.

Design: Laboratory-based study.

Setting: Research laboratory in a university hospital.

Patient(s): One hundred men attending for diagnostic semen analysis as part of infertility investigations and three sperm donors providing ejaculates for research purposes.

Main Outcome Measure(s): Number of DNA copies of C. trachomatis, infectivity in an HeLa cell monolayer, and immunofluorescence.

Result(s): Of the 100 semen samples examined, 13 contained detectable levels of C. trachomatis DNA (675–15,920 copies/mL) and in only 7 was this completely removed after sperm washing. In the remaining six DNA-positive samples, the number of copies in the postwash preparation ranged from 36–455 per mL. Experimental inoculations found that postwash preparations containing C. trachomatis DNA as low as 61 copies/mL were able to establish an infection in vitro.

Conclusion(s): Undiagnosed C. trachomatis infections in men attending for assisted conception could potentially lead to infection or contamination of the IVF culture system as sperm washing methods are not 100% effective.

Item Type: Article
Research Institute, Centre or Group: Biomedical Research Centre
Identification Number: 10.1016/j.fertnstert.2008.08.128
Depositing User: Sarah Ward
Date Deposited: 13 May 2010 09:49
Last Modified: 13 May 2010 09:49
URI: http://shura.shu.ac.uk/id/eprint/1898

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