AL RUBAYE, Ali, NABOK, Aleksey, CATANANTE, Gaelle, MARTY, Jean-Louis, TAKACS, Ezster and SZEKACS, Andras (2018). Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry. Sensors and Actuators B: Chemical, 263, 248-251.
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Abstract
The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label-free optical detection of OTA down to 0.01 ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant KD = 1.8 10−8 Mol which is characteristic for highly specific aptamer/OTA binding. © 2018
Item Type: | Article |
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Research Institute, Centre or Group - Does NOT include content added after October 2018: | Materials and Engineering Research Institute > Advanced Coatings and Composites Research Centre > Electronic Materials and Sensors Research Group |
Identification Number: | https://doi.org/10.1016/j.snb.2018.01.220 |
Page Range: | 248-251 |
Depositing User: | Carmel House |
Date Deposited: | 13 Mar 2018 11:32 |
Last Modified: | 18 Mar 2021 06:35 |
URI: | https://shura.shu.ac.uk/id/eprint/18924 |
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