Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

AL RUBAYE, Ali, NABOK, Aleksey, CATANANTE, Gaelle, MARTY, Jean-Louis, TAKACS, Ezster and SZEKACS, Andras (2018). Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry. Sensors and Actuators B: Chemical, 263, 248-251.

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Link to published version:: https://doi.org/10.1016/j.snb.2018.01.220

Abstract

The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label-free optical detection of OTA down to 0.01 ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant KD = 1.8 10−8 Mol which is characteristic for highly specific aptamer/OTA binding. © 2018

Item Type: Article
Research Institute, Centre or Group: Materials and Engineering Research Institute > Thin Films Research Centre > Electronic Materials and Sensors Research Group
Identification Number: https://doi.org/10.1016/j.snb.2018.01.220
Depositing User: Carmel House
Date Deposited: 13 Mar 2018 11:32
Last Modified: 25 Apr 2018 23:25
URI: http://shura.shu.ac.uk/id/eprint/18924

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