Fatty acid supplementation reverses the small colony variant phenotype in triclosan-adapted staphylococcus aureus: Genetic, proteomic and phenotypic analyses

BAZAID, Abdulrahman S, FORBES, Sarah, HUMPHREYS, Gavin J, LEDDER, Ruth G, O'CUALAIN, Ronan and MCBAIN, Andrew J (2018). Fatty acid supplementation reverses the small colony variant phenotype in triclosan-adapted staphylococcus aureus: Genetic, proteomic and phenotypic analyses. Scientific Reports, 8 (1), p. 3876.

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Abstract

Staphylococcus aureus can develop a small colony variant (SCV) phenotype in response to sub-lethal exposure to the biocide triclosan. In the current study, whole genome sequencing was performed and changes in virulence were investigated in five Staphylococcus aureus strains following repeated exposure to triclosan. Following exposure, 4/5 formed SCV and exhibited point mutations in the triclosan target gene fabI with 2/4 SCVs showing mutations in both fabI and fabD. The SCV phenotype was in all cases immediately reversed by nutritional supplementation with fatty acids or by repeated growth in the absence of triclosan, although fabI mutations persisted in 3/4 reverted SCVs. Virulence, determined using keratinocyte invasion and Galleria mellonella pathogenicity assays was significantly (p < 0.05) attenuated in 3/4 SCVs and in the non-SCV triclosan-adapted bacterium. Proteomic analysis revealed elevated FabI in 2/3 SCV and down-regulation in a protein associated with virulence in 1/3 SCV. In summary, attenuated keratinocyte invasion and larval virulence in triclosan-induced SCVs was associated with decreases in growth rate and virulence factor expression. Mutation occurred in fabI, which encodes the main triclosan target in all SCVs and the phenotype was reversed by fatty acid supplementation, demonstrating an association between fatty acid metabolism and triclosan-induced SCV.

Item Type:	Article
Additional Information:	** From PubMed via Jisc Publications Router. ** History: received 05-10-2017; accepted 06-02-2018.
Research Institute, Centre or Group - Does NOT include content added after October 2018:	Biomedical Research Centre
Identification Number:	https://doi.org/10.1038/s41598-018-21925-6
Page Range:	p. 3876
SWORD Depositor:	Margaret Boot
Depositing User:	Margaret Boot
Date Deposited:	13 Mar 2018 12:01
Last Modified:	18 Mar 2021 15:21
URI:	https://shura.shu.ac.uk/id/eprint/18918

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