REID, M. J., CROSS, A. K., HADDOCK, G., ALLAN, S. M., STOCK, C. J., WOODROOFE, M. N., BUTTLE, D. J. and BUNNING, R. A. D. (2009). ADAMTS-9 expression is up-regulated following transient middle cerebral artery occlusion (tMCAo) in the rat. Neuroscience letters, 452 (3), 252-257.Full text not available from this repository.
The ADAMTS enzymes (a disintegrin and metalloproteinase with thrombospondin type 1-like motifs) have important roles in central nervous system (CNS) physiology and pathology. This current study aimed to analyse the expression of ADAMTS-9 following transient middle cerebral artery occlusion (tMCAo) in the rat, a model of focal cerebral ischaemia. Using real-time RT-PCR, ADAMTS-9 mRNA was demonstrated to be significantly up-regulated in tMCAo brain tissue compared to sham-operated at 24 h post-ischaemia. The mature form of the ADAMTS-9 protein was only detected by Western blotting in brains subjected to tMCAo at 24 h. In situ hybridisation demonstrated that ADAMTS-9 mRNA was expressed by neurones in tMCAo tissue. This study indicates that ADAMTS-9 expression is modulated in response to cerebral ischaemia in vivo and further research will resolve whether it plays a role in the subsequent degenerative or repair processes.
|Research Institute, Centre or Group:||Biomedical Research Centre|
|Depositing User:||Sarah Ward|
|Date Deposited:||12 May 2010 09:37|
|Last Modified:||12 May 2010 09:38|
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