ADAMTS-9 expression is up-regulated following transient middle cerebral artery occlusion (tMCAo) in the rat

REID, M. J., CROSS, A. K., HADDOCK, G., ALLAN, S. M., STOCK, C. J., WOODROOFE, M. N., BUTTLE, D. J. and BUNNING, R. A. D. (2009). ADAMTS-9 expression is up-regulated following transient middle cerebral artery occlusion (tMCAo) in the rat. Neuroscience letters, 452 (3), 252-257.

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Link to published version:: 10.1016/j.neulet.2009.01.058

Abstract

The ADAMTS enzymes (a disintegrin and metalloproteinase with thrombospondin type 1-like motifs) have important roles in central nervous system (CNS) physiology and pathology. This current study aimed to analyse the expression of ADAMTS-9 following transient middle cerebral artery occlusion (tMCAo) in the rat, a model of focal cerebral ischaemia. Using real-time RT-PCR, ADAMTS-9 mRNA was demonstrated to be significantly up-regulated in tMCAo brain tissue compared to sham-operated at 24 h post-ischaemia. The mature form of the ADAMTS-9 protein was only detected by Western blotting in brains subjected to tMCAo at 24 h. In situ hybridisation demonstrated that ADAMTS-9 mRNA was expressed by neurones in tMCAo tissue. This study indicates that ADAMTS-9 expression is modulated in response to cerebral ischaemia in vivo and further research will resolve whether it plays a role in the subsequent degenerative or repair processes.

Item Type: Article
Research Institute, Centre or Group: Biomedical Research Centre
Identification Number: 10.1016/j.neulet.2009.01.058
Depositing User: Sarah Ward
Date Deposited: 12 May 2010 10:37
Last Modified: 12 May 2010 10:38
URI: http://shura.shu.ac.uk/id/eprint/1873

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