Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates

LOCK, Malcolm, NICHOL, Tim, MURRELL, J Colin and SMITH, Thomas (2017). Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates. FEMS microbiology letters, 364 (13), fnx137.

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Official URL: https://academic.oup.com/femsle/article-lookup/doi...
Link to published version:: 10.1093/femsle/fnx137

Abstract

Soluble methane monooxygenase (sMMO) from methane-oxidising bacteria can oxygenate more than 100 hydrocarbons and is one of the most catalytically versatile biological oxidation catalysts. Expression of recombinant sMMO has to date not been achieved in Escherichia coli and so an alternative expression system must be used to manipulate it genetically. Here we report substantial improvements to the previously described system for mutagenesis of sMMO and expression of recombinant enzymes in a methanotroph (Methylosinus trichosporium OB3b) expression system. This system has been utilised to make a number of new mutants and to engineer sMMO to increase its catalytic precision with a specific substrate whilst increasing activity by up to 6-fold. These results are the first 'proof-of-principle' experiments illustrating the feasibility of developing sMMO-derived catalysts for diverse applications. [Abstract copyright: © FEMS 2017.]

Item Type: Article
Additional Information: ** From PubMed via Jisc Publications Router. ** History: ** received: 03-04-2017 ** accepted: 27-06-2017
Uncontrolled Keywords: biocatalysis, hydrocarbon oxidation, methane, monooxygenase, protein engineering
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Identification Number: 10.1093/femsle/fnx137
SWORD Depositor: Jill Hazard
Depositing User: Jill Hazard
Date Deposited: 11 Sep 2017 15:48
Last Modified: 12 Sep 2017 07:21
URI: http://shura.shu.ac.uk/id/eprint/16693

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