Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis

STEINBERG, Julia, RITCHIE, Graham R.S., ROUMELIOTIS, Theodoros I., JAYASURIYA, Raveen L., CLARK, Matthew J., BROOKS, Roger A., BINCH, Abbie L.A., SHAH, Karan M., COYLE, Rachael, PARDO, Mercedes, LE MAITRE, Christine, RAMOS, Yolande F.M., NELISSEN, Rob G.H.H., MEULENBELT, Ingrid, MCCASKIE, Andrew W, CHOUDHARY, Jyoti S., WILKINSON, J. Mark and ZEGGINI, Eleftheria (2017). Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis. Scientific reports, 7 (1), p. 8935.

[img]
Preview
PDF
LeMaitre-IntegrativeEpigenomicsTranscriptomicsandProteomics(VoR).pdf - Published Version
Creative Commons Attribution.

Download (2MB) | Preview
Official URL: http://www.nature.com/articles/s41598-017-09335-6
Link to published version:: https://doi.org/10.1038/s41598-017-09335-6
Related URLs:

    Abstract

    Osteoarthritis (OA) is a common disease characterized by cartilage degeneration and joint remodeling. The underlying molecular changes underpinning disease progression are incompletely understood. We investigated genes and pathways that mark OA progression in isolated primary chondrocytes taken from paired intact versus degraded articular cartilage samples across 38 patients undergoing joint replacement surgery (discovery cohort: 12 knee OA, replication cohorts: 17 knee OA, 9 hip OA patients). We combined genome-wide DNA methylation, RNA sequencing, and quantitative proteomics data. We identified 49 genes differentially regulated between intact and degraded cartilage in at least two -omics levels, 16 of which have not previously been implicated in OA progression. Integrated pathway analysis implicated the involvement of extracellular matrix degradation, collagen catabolism and angiogenesis in disease progression. Using independent replication datasets, we showed that the direction of change is consistent for over 90% of differentially expressed genes and differentially methylated CpG probes. AQP1, COL1A1 and CLEC3B were significantly differentially regulated across all three -omics levels, confirming their differential expression in human disease. Through integration of genome-wide methylation, gene and protein expression data in human primary chondrocytes, we identified consistent molecular players in OA progression that replicated across independent datasets and that have translational potential.

    Item Type: Article
    Additional Information: ** From PubMed via Jisc Publications Router. ** History: ** received: 20-04-2017 ** accepted: 17-07-2017
    Research Institute, Centre or Group - Does NOT include content added after October 2018: Biomolecular Sciences Research Centre
    Identification Number: https://doi.org/10.1038/s41598-017-09335-6
    Page Range: p. 8935
    SWORD Depositor: Jill Hazard
    Depositing User: Jill Hazard
    Date Deposited: 04 Sep 2017 08:45
    Last Modified: 08 Jul 2019 18:31
    URI: http://shura.shu.ac.uk/id/eprint/16630

    Actions (login required)

    View Item View Item

    Downloads

    Downloads per month over past year

    View more statistics