MALDI-MSI of lipids in human skin

HART, Philippa J. and CLENCH, Malcolm R. (2017). MALDI-MSI of lipids in human skin. In: COLE, Laura, (ed.) Imaging mass spectrometry : methods and protocols. Methods in Molecular Biology (1618). New York, Humana Press, 29-36.

Full text not available from this repository.
Official URL: https://link.springer.com/protocol/10.1007%2F978-1...
Link to published version:: 10.1007/978-1-4939-7051-3_4

Abstract

Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of skin. This includes information on the distribution of pharmaceuticals following topical treatments, through to the examination of the composition of different skin layers and studies of proteomic, lipidomic, and metabolomic responses to disease, wounds, and external stimuli. Here, we describe the handling procedures, preparatory treatment, and mass spectrometry setup required for the MALDI MSI analysis of lipids within human skin samples

Item Type: Book Section
Additional Information: Book series ISSN: 1064-3745
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Identification Number: 10.1007/978-1-4939-7051-3_4
Depositing User: Carmel House
Date Deposited: 18 Jul 2017 14:45
Last Modified: 18 Jul 2017 14:45
URI: http://shura.shu.ac.uk/id/eprint/15914

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics