Tools
Tools
HUANG, Jin, QIN, Hao, YANG, Yihua, CHEN, Xiaoyan, ZHANG, Jiamiao, LAIRD, Susan, WANG, Chi Chiu, CHAN, Ting Fung and LI, Tin Chiu (2017). Endometrial transcriptome in recurrent miscarriage and recurrent implantation failure. Reproduction, 153 (6).
|
PDF (Article)
Laird - Endometrial transcriptome in recurrent miscarriage (AM - article).pdf - Accepted Version All rights reserved. Download (607kB) | Preview |
|
|
PDF (Table)
Laird - Endometrial transcriptome in recurrent miscarriage (AM - tables).pdf - Supplemental Material All rights reserved. Download (9kB) | Preview |
|
|
PDF (Figure 1)
Laird - Endometrial transcriptome in recurrent miscarriage (AM - fig1).pdf - Supplemental Material All rights reserved. Download (120kB) | Preview |
|
|
PDF (Figure 2)
Laird - Endometrial transcriptome in recurrent miscarriage (AM - fig2).pdf - Supplemental Material All rights reserved. Download (171kB) | Preview |
|
|
PDF (Figure 3)
Laird - Endometrial transcriptome in recurrent miscarriage (AM - fig3).pdf - Supplemental Material All rights reserved. Download (423kB) | Preview |
|
|
PDF (Figure 4)
Laird - Endometrial transcriptome in recurrent miscarriage (AM - fig4).pdf - Supplemental Material All rights reserved. Download (138kB) | Preview |
|
|
PDF (Figure 5)
Laird - Endometrial transcriptome in recurrent miscarriage (AM - fig5).pdf - Supplemental Material All rights reserved. Download (97kB) | Preview |
|
|
PDF (Figure 6)
Laird - Endometrial transcriptome in recurrent miscarriage (AM - fig6).pdf - Supplemental Material All rights reserved. Download (155kB) | Preview |
Abstract
The endometrium becomes receptive to the embryo only in the mid-luteal phase, but not other stages of the menstrual cycle. Endometrial factors play an important role in implantation. Women with recurrent miscarriage and recurrent implantation failure have both been reported to have altered expression of receptivity markers during the window of implantation. We aimed to compare the gene expression profiles of the endometrium in the window of implantation among women with unexplained recurrent implantation failures (RIF) and unexplained recurrent miscarriages (RM) by RNA sequencing (RNA-Seq). In total 20 patients (9 RIF and 11 RM) were recruited. In addition 4 fertile subjects were included as reference. Endometrium samples were precisely timed on the 7th day after luteal hormone surge (LH+7). All the 24 samples were extracted for total RNA. The transcriptome was determined by RNA-Seq in first 14 RNA samples (5 RIF, 6 RM, and 3 fertile). Differentially expressed genes between RM and RIF were validated by quantitative real time PCR (qPCR) in all 24 RNA samples (9 RIF, 11 RM and 4 fertile). Complementary and coagulation cascades pathway was the significantly up-regulated in RIF while down-regulated in RM. Differentially expressed genes C3, C4, C4BP, DAF, DF and SERPING1 in complement and coagulation cascade pathway between RM and RIF were further validated by qPCR. This study identified differential molecular pathways in endometrium between RIF and RM, which potentially affect the implantation process.
| Item Type: | Article |
|---|---|
| Additional Information: | ** From PubMed via Jisc Publications Router. ** History: ** received: 18-10-2016 ** revised: 02-03-2017 ** accepted: 09-03-2017 |
| Research Institute, Centre or Group - Does NOT include content added after October 2018: | Biomedical Research Centre |
| Identification Number: | https://doi.org/10.1530/REP-16-0574 |
| SWORD Depositor: | Helen Garner |
| Depositing User: | Helen Garner |
| Date Deposited: | 16 May 2017 13:43 |
| Last Modified: | 18 Mar 2021 11:16 |
| URI: | https://shura.shu.ac.uk/id/eprint/15428 |
Actions (login required)
 |
View Item |
Downloads
Downloads per month over past year