Hybridization of Genomic DNA Adsorbed Electrostatically onto Cationic Surfaces

NABOK, A. V., TSARGORODSKAYA, A., GAUTHIER, D., DAVIS, F., HIGSON, S. P. J., BERZINA, T., CRISTOFOLINI, L. and FONTANA, M. P. (2009). Hybridization of Genomic DNA Adsorbed Electrostatically onto Cationic Surfaces. The Journal of Physical Chemistry B, 113 (22), 7897-7902.

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Link to published version:: https://doi.org/10.1021/jp9010636
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    Our previous study revealed an intriguing phenomenon of partial hybridization of two single strands of genomic C, DNA, with one of them being electrostatically adsorbed on a solid surface. Although the effect was confirmed with different methods and even recommended for a crude DNA analysis, the exact mechanism of hybridization was not clear. This work presents the results of more detailed study of adsorption and hybridization of two genomic DNA, of salmon and herring, using the experimental techniques of total internal reflection ellipsometry (TIRE), ATR FTIR spectroscopy, and AFM. The in situ TIRE study of the hybridization kinetics allowed the evaluation of the association constant. It appeared to be in the range of 10(5) mol(-1) L for binding complementary ss-DNA in comparison to 10(4) mol(-1) L for binding of noncomplementary ss-DNA. FTIR study directly confirmed the effect of partial binding of complementary ss-DNA by monitoring the 1650 and 1690 cm(-1) spectral bands. AFM showed the transformation from clearly resolved images of separate chains of ss-DNA molecules adsorbed on the surface of mica to an inhomogeneous layer of tangled and overlapping DNA molecules following binding of another complementary ss-DNA.

    Item Type: Article
    Research Institute, Centre or Group - Does NOT include content added after October 2018: Materials and Engineering Research Institute > Thin Films Research Centre > Electronic Materials and Sensors Research Group
    Identification Number: https://doi.org/10.1021/jp9010636
    Page Range: 7897-7902
    Depositing User: Ann Betterton
    Date Deposited: 31 Mar 2010 15:45
    Last Modified: 18 Mar 2021 10:00
    URI: http://shura.shu.ac.uk/id/eprint/1442

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