Subchronic administration of phencyclidine produces hypermethylation in the parvalbumin gene promoter in rat brain

FACHIM, Helenne, SRISAWAT, Umarat, DALTON, Caroline, HARTE, Michael K, MARSH, Samuel, NEILL, Joanna C and REYNOLDS, Gavin (2016). Subchronic administration of phencyclidine produces hypermethylation in the parvalbumin gene promoter in rat brain. Epigenomics, 8 (9), 1179-1183.

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Official URL: http://www.futuremedicine.com/doi/abs/10.2217/epi-...
Link to published version:: 10.2217/epi-2016-0050

Abstract

Aim: A deficit in parvalbumin neurons is found in schizophrenia and several animal models of the disease. In this preliminary study, we determined whether one such model, phencyclidine (PCP) administration, results in changes in DNA methylation in the rat Pvalb promoter. Materials & methods: DNA from hippocampus and prefrontal cortex from rats, which 6 weeks previously received either 2 mg/kg PCP or vehicle for 7 days, underwent bisulphite pyrosequencing to determine methylation. Results: PCP administration induced significantly greater methylation at one of two Pvalb CpG sites in both prefrontal cortex and hippocampus, while no significant difference was found in long interspersed nucleotide element-1, a global measure of DNA methylation. Conclusion: Subchronic PCP administration results in a specific hypermethylation in the Pvalb promoter which may contribute to parvalbumin deficits in this animal model of psychosis.

Item Type: Article
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Identification Number: 10.2217/epi-2016-0050
Depositing User: Marguerite Lyons
Date Deposited: 19 Sep 2016 09:41
Last Modified: 03 Dec 2016 00:23
URI: http://shura.shu.ac.uk/id/eprint/13534

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