RIMMER, Stephen, JOHNSON, Claire, ZHAO, Bojun, COLLIER, Joyleen, GILMORE, Louisa, SABNIS, Subodh, WYMAN, Paul, SAMMON, Chris, FULLWOOD, Nigel J. and MACNEIL, Sheila (2007). Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. Biomaterials, 28 (35), 5319 - 5331.
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The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl α–ω diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium. Keywords: Epithelial cells
|Research Institute, Centre or Group:||Materials and Engineering Research Institute > Polymers Nanocomposites and Modelling Research Centre > Polymers, Composites and Spectroscopy Group|
|Depositing User:||Chris Sammon|
|Date Deposited:||26 Aug 2016 12:33|
|Last Modified:||19 Oct 2016 22:19|
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