Temocillin: a new candidate antibiotic for local antimicrobial delivery in orthopaedic surgery?

BARKER, Stewart, NICHOL, Tim, HARRISON, Patrick L., STOCKLEY, Ian, TOWNSEND, Robert and SMITH, Thomas J. (2015). Temocillin: a new candidate antibiotic for local antimicrobial delivery in orthopaedic surgery? The Journal of antimicrobial chemotherapy, 70 (3), 780-783.

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Official URL: http://dx.doi.org/10.1093/jac/dku425
Link to published version:: 10.1093/jac/dku425


Objectives - To assess the performance of the Gram-negative-specific antibiotic temocillin in polymethylmethacrylate bone cement pre-loaded with gentamicin, as a strategy for local antibiotic delivery.

Methods - Temocillin was added at varying concentrations to commercial gentamicin-loaded bone cement. The elution of the antibiotic from cement samples over a 2 week period was quantified by LC-MS. The eluted temocillin was purified by fast protein liquid chromatography and the MICs for a number of antibiotic-resistant Escherichia coli were determined. The impact strength of antibiotic-loaded samples was determined using a Charpy-type impact testing apparatus.

Results - LC-MS data showed temocillin eluted to clinically significant concentrations within 1 h in this laboratory system and the eluted temocillin retained antimicrobial activity against all organisms tested. Impact strength analysis showed no significant difference between cement samples with or without temocillin.

Conclusions - Temocillin can be added to bone cement and retains its antimicrobial activity after elution. The addition of up to 10% temocillin did not affect the impact strength of the cement. The results show that temocillin is a promising candidate for use in antibiotic-loaded bone cement.

Item Type: Article
Additional Information: First published online: October 17, 2014
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Identification Number: 10.1093/jac/dku425
Depositing User: Marguerite Lyons
Date Deposited: 26 Jun 2015 10:36
Last Modified: 19 Oct 2016 22:54
URI: http://shura.shu.ac.uk/id/eprint/10560

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