BARKER, Stewart, NICHOL, Tim, HARRISON, Patrick L., STOCKLEY, Ian, TOWNSEND, Robert and SMITH, Thomas J. (2015). Temocillin: a new candidate antibiotic for local antimicrobial delivery in orthopaedic surgery? The Journal of antimicrobial chemotherapy, 70 (3), 780-783.
Smith_-_Temocillin_paper_final.pdf - Accepted Version
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Objectives - To assess the performance of the Gram-negative-specific antibiotic temocillin in polymethylmethacrylate bone cement pre-loaded with gentamicin, as a strategy for local antibiotic delivery.
Methods - Temocillin was added at varying concentrations to commercial gentamicin-loaded bone cement. The elution of the antibiotic from cement samples over a 2 week period was quantified by LC-MS. The eluted temocillin was purified by fast protein liquid chromatography and the MICs for a number of antibiotic-resistant Escherichia coli were determined. The impact strength of antibiotic-loaded samples was determined using a Charpy-type impact testing apparatus.
Results - LC-MS data showed temocillin eluted to clinically significant concentrations within 1 h in this laboratory system and the eluted temocillin retained antimicrobial activity against all organisms tested. Impact strength analysis showed no significant difference between cement samples with or without temocillin.
Conclusions - Temocillin can be added to bone cement and retains its antimicrobial activity after elution. The addition of up to 10% temocillin did not affect the impact strength of the cement. The results show that temocillin is a promising candidate for use in antibiotic-loaded bone cement.
|Additional Information:||First published online: October 17, 2014|
|Research Institute, Centre or Group:||Biomolecular Sciences Research Centre|
|Depositing User:||Marguerite Lyons|
|Date Deposited:||26 Jun 2015 10:36|
|Last Modified:||19 Oct 2016 22:54|
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