ROY, Sumita, PHANSOPA, Chatchawal, STAFFORD, Prachi, HONMA, Kiyonobu, DOUGLAS, C W Ian, SHARMA, Ashu and STAFFORD, Graham P (2012). Beta-hexosaminidase activity of the oral pathogen Tannerella forsythia influences biofilm formation on glycoprotein substrates. FEMS Immunology and Medical Microbiology, 65 (1), 116-120.Full text not available from this repository.
Tannerella forsythia is an important pathogen in periodontal disease. Previously, we showed that its sialidase activity is key to utilization of sialic acid from a range of human glycoproteins for biofilm growth and initial adhesion. Removal of terminal sialic acid residues often exposes β-linked glucosamine or galactosamine, which may also be important adhesive molecules. In turn, these residues are often removed by a group of enzymes known as β-hexosaminidases. We show here that T. forsythia has the ability to cleave glucosamine and galactosamine from model substrates and that this activity can be inhibited by the hexosaminidase inhibitor PugNAc (O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino N-phenyl carbamate). We now demonstrate for the first time that β-hexosaminidase activity plays a role in biofilm growth on glycoprotein-coated surfaces because biofilm growth and initial cell adhesion are inhibited by PugNAc. In contrast, adhesion to siallo-glycoprotein-coated surfaces is unaltered by PugNAc in the absence of sialidase activity (using a sialidase-deficient mutant) or surprisingly on the clinically relevant substrates saliva or serum. These data indicate that β-hexosaminidase activity has a significant role in biofilm formation in combination with sialidase activity in the biofilm lifestyle of T. forsythia.
|Additional Information:||Article first published online: 14 FEB 2012|
|Research Institute, Centre or Group:||Biomolecular Sciences Research Centre|
|Depositing User:||Jamie Young|
|Date Deposited:||03 Jun 2015 13:20|
|Last Modified:||03 Jun 2015 13:20|
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