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LIU, Gaomin, YANG, Fan, LI, Fangfang, LI, Zhongjie, LANG, Yange, SHEN, Bingzheng, WU, Yingliang, LI, Wenxin, HARRISON, Patrick L., STRONG, Peter N., XIE, Yingqiu, MILLER, Keith and CAO, Zhijian (2018). Therapeutic potential of a scorpion venom-derived antimicrobial peptide and its homologs against antibiotic-resistant gram-positive bacteria. Frontiers in Microbiology, 9, p. 1159.
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Abstract
The alarming rise in the prevalence of antibiotic resistance among pathogenic bacteria poses a unique challenge for the development of effective therapeutic agents. Antimicrobial peptides (AMPs) have attracted a great deal of attention as a possible solution to the increasing problem of antibiotic-resistant bacteria. Marcin-18 was identified from the scorpion Mesobuthus martensii at both DNA and protein levels. The genomic sequence revealed that the marcin-18 coding gene contains a phase-I intron with a GT-AG splice junction located in the DNA region encoding the N-terminal part of signal peptide. The peptide marcin-18 was also isolated from scorpion venom. A protein sequence homology search revealed that marcin-18 shares extremely high sequence identity to the AMPs meucin-18 and megicin-18. In vitro, chemically synthetic marcin-18 and its homologs (meucin-18 and megicin-18) showed highly potent inhibitory activity against Gram-positive bacteria, including some clinical antibiotic-resistant strains. Importantly, in a mouse acute peritonitis model, these peptides significantly decreased the bacterial load in ascites and rescued nearly all mice heavily infected with clinical methicillin-resistant Staphylococcus aureus from lethal bacteremia. Peptides exerted antimicrobial activity via a bactericidal mechanism and killed bacteria through membrane disruption. Taken together, marcin-18 and its homologs have potential for development as therapeutic agents for treating antibiotic-resistant, Gram-positive bacterial infections
| Item Type: | Article |
|---|---|
| Departments - Does NOT include content added after October 2018: | Health and Well-being > Department of Bioscience |
| Identification Number: | https://doi.org/10.3389/fmicb.2018.01159 |
| Page Range: | p. 1159 |
| Depositing User: | Carmel House |
| Date Deposited: | 12 Jun 2018 11:44 |
| Last Modified: | 17 Mar 2021 17:16 |
| URI: | https://shura.shu.ac.uk/id/eprint/21557 |
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