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CHEN, Yu-Su. (2014). Phosphonium-functionalised gold nanoparticles for mitochondria targeted therapeutics. Doctoral, Sheffield Hallam University (United Kingdom)..
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Abstract
The work presented in this thesis demonstrates that triarylphosphoniopropyl-thiosulfate zwitterions and w-thioacetylpropyl(triphenyl)phosphonium salts can be used to prepare cationic, water-soluble gold nanoparticles with mean core sizes in the range of 2.5-5 nm. Phosphonium-functionalised gold nanoparticles have been characterised by a number of techniques including NMR, LDI-MS, SIMS, XPS, TGA, ICP-MS, MALDI-MS and TEM.Cytotoxicity studies illustrated that phosphonium ligands are relatively non-toxic to human prostate cancer cells and therefore can be used as a delivery vector to delivery gold nanoparticles specifically to the site of the mitochondria for other therapeutic applications such as photothermal therapy. Cellular uptake studies of phosphonium ligands by MALDI-MS showed that they are rapidly taken-up by cells within ten minutes.Phosphonium-functionalised gold nanoparticles are soluble in biological media which is of great importance for cell biology studies. Initial photothermal therapy studies demonstrated that the gold nanoparticles responds specifically to a green light excitation source (510-550 nm) which overlaps the surface plasmon resonance band of the phosphonium-functionalised gold nanoparticles at 525 nm. Preliminary data also showed that phosphonium-functionalised gold nanoparticles can selectively induce apoptosis in cells followed by irradiation, this was confirmed by Hoechst and caspase-3 staining. Quantification studies of phosphonium-functionalised gold nanoparticles by ICP-MS illustrated that these nanoparticle have good uptake in cells (above 75%). TEM data confirmed that phosphonium-functionalised gold nanoparticles are taken-up by human prostate cancer cells and are localised in the mitochondria.
| Item Type: | Thesis (Doctoral) |
|---|---|
| Contributors: | Thesis advisor - Bricklebank, Neil [0000-0002-1614-2260] Thesis advisor - Cross, Neil [0000-0003-2055-5815] |
| Additional Information: | Thesis (Ph.D.)--Sheffield Hallam University (United Kingdom), 2014. |
| Research Institute, Centre or Group - Does NOT include content added after October 2018: | Sheffield Hallam Doctoral Theses |
| Depositing User: | EPrints Services |
| Date Deposited: | 10 Apr 2018 17:19 |
| Last Modified: | 03 May 2023 02:05 |
| URI: | https://shura.shu.ac.uk/id/eprint/19454 |
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