Elongated oligomers in β2-microglobulin amyloid assembly revealed by ion mobility spectrometry-mass spectrometry

SMITH, D. P., RADFORD, S. E. and ASHCROFT, A. E. (2010). Elongated oligomers in β2-microglobulin amyloid assembly revealed by ion mobility spectrometry-mass spectrometry. Proceedings of the National Academy of Sciences, 107 (15), 6794-6798.

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Abstract

The key to understanding amyloid disease is the characterization of oligomeric species formed during the early stages of fibril assembly. Here we have used electrospray ionisation-ion mobility spectrometry-mass spectrometry to identify and structurally characterize the oligomers formed during amyloid assembly from β2-microglobulin (β2m). β2m oligomers are shown to have collision cross-sections consistent with monomeric units arranged in elongated assemblies prior to fibril formation. Direct observation, separation, and quantification of transient oligomeric species reveals that monomers to tetramers are populated through the lag phase with no evidence for the significant population of larger oligomeric species under the conditions employed. The dynamics of each oligomeric species were monitored directly within the ensemble at concentrations commensurate with amyloid formation by observing the subunit exchange of 14N- and 15N- labeled oligomers. Analysis of the data revealed a decrease in oligomer dynamics concomitant with increasing oligomer size and the copopulation of dynamic dimeric and trimeric species with more stable trimeric and tetrameric species. The results presented map the events occurring during the lag phase of fibril formation and give a clear insight into the structural characteristics and dynamic nature of the β2m oligomers, demonstrating the existence of elongated assemblies arising from an intact amyloidogenic protein during fibril formation.

Item Type:	Article
Research Institute, Centre or Group - Does NOT include content added after October 2018:	Biomedical Research Centre
Identification Number:	https://doi.org/10.1073/pnas.0913046107
Page Range:	6794-6798
Depositing User:	Users 4 not found.
Date Deposited:	12 May 2010 09:01
Last Modified:	18 Mar 2021 21:15
URI:	https://shura.shu.ac.uk/id/eprint/1869

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