Antigen retrieval prior to on-tissue digestion of formalin-fixed paraffin-embedded tumour tissue sections yields oxidation of proline residues.

DJIDJA, Marie-Claude, CLAUDE, Emmanuelle, SCRIVEN, Peter, ALLEN, David, CAROLAN, Vikki and CLENCH, Malcolm (2016). Antigen retrieval prior to on-tissue digestion of formalin-fixed paraffin-embedded tumour tissue sections yields oxidation of proline residues. Biochimica et biophysica acta, 1865 (7), 901-906.

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Abstract

MALDI-mass spectrometry imaging (MALDI-MSI) has been shown to allow the study of protein distribution and identification directly within formalin-fixed paraffin-embedded (FFPE) tissue sections. However, direct protein identification from tissue sections remains challenging due to signal interferences and/or existing post-translational or other chemical modifications. The use of antigen retrieval (AR) has been demonstrated for unlocking proteins prior to in situ enzymatic digestion and MALDI-MSI analysis of FFPE tissue sections. In the work reported here, the identification of proline oxidation, which may occur when performing the AR protocol, is described. This facilitated and considerably increased the number of identified peptides when adding proline oxidation as a variable modification to the MASCOT search criteria. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.

Item Type:	Article
Research Institute, Centre or Group - Does NOT include content added after October 2018:	Biomedical Research Centre
Identification Number:	https://doi.org/10.1016/j.bbapap.2016.11.019
Page Range:	901-906
SWORD Depositor:	Helen Garner
Depositing User:	Helen Garner
Date Deposited:	20 Jan 2017 13:00
Last Modified:	18 Mar 2021 01:03
URI:	https://shura.shu.ac.uk/id/eprint/14372

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