Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

RIMMER, Stephen, JOHNSON, Claire, ZHAO, Bojun, COLLIER, Joyleen, GILMORE, Louisa, SABNIS, Subodh, WYMAN, Paul, SAMMON, Chris, FULLWOOD, Nigel J. and MACNEIL, Sheila (2007). Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. Biomaterials, 28 (35), 5319 - 5331.

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Abstract

The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl α–ω diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium. Keywords: Epithelial cells

Item Type:	Article
Research Institute, Centre or Group - Does NOT include content added after October 2018:	Materials and Engineering Research Institute > Advanced Coatings and Composites Research Centre > Polymers, Composites and Spectroscopy Group
Identification Number:	https://doi.org/10.1016/j.biomaterials.2007.08.028
Page Range:	5319 - 5331
Depositing User:	Chris Sammon
Date Deposited:	26 Aug 2016 12:33
Last Modified:	18 Mar 2021 04:48
URI:	https://shura.shu.ac.uk/id/eprint/12664

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