Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

RIMMER, S., JOHNSON, C., ZHAO, B. and SAMMON, C. (2007). Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. Biomaterials.

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Link to published version:: 10.1016/j.biomaterials.2007.08.028


The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl –ω diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium.

Item Type: Article
Additional Information: Copyright © Elsevier Ltd
Research Institute, Centre or Group: Materials and Engineering Research Institute > Polymers Nanocomposites and Modelling Research Centre > Polymers, Composites and Spectroscopy Group
Identification Number: 10.1016/j.biomaterials.2007.08.028
Depositing User: Ann Betterton
Date Deposited: 29 Jul 2008
Last Modified: 13 Jun 2017 12:45
URI: http://shura.shu.ac.uk/id/eprint/943

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