Effects of cannabinoids on nitric oxide production by chondrocytes and proteoglycan degradation in cartilage

MBVUNDULA, E. C., BUNNING, R. A. D. and RAINSFORD, K. D. (2004). Effects of cannabinoids on nitric oxide production by chondrocytes and proteoglycan degradation in cartilage. Biochemical pharmacology, 69 (4), 635-640.

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Link to published version:: 10.1016/j.bcp.2004.11.018

Abstract

Cannabinoids have been reported to have anti-inflammatory effects and reduce joint damage in animal models of arthritis. This suggests a potential therapeutic role in arthritis of this group of compounds. Cannabinoids were studied to determine whether they have direct effects on chondrocyte metabolism resulting in cartilage protection. Synthetic cannabinoids, R-(+)-Win-55,212 (Win-2) and S-(−)-Win-55,212 (Win-3) and the endocannabinoid, anandamide, were investigated on unstimulated or IL-1-stimulated nitric oxide (NO) production in bovine articular chondrocytes as well as on cartilage proteoglycan breakdown in bovine nasal cartilage explants. Win-2 significantly inhibited (P < 0.05) NO production in chondrocytes at 1–10 μM concentrations. The combined CB1 and CB2 cannabinoid receptor antagonists, AM281 and AM630, respectively, at 100 μM did not block this effect, but instead they potentiated it. Anandamide and Win-2 (5–50 μM) also inhibited the release of sulphated glycosaminoglycans in bovine cartilage explants. The results suggest that some cannabinoids may prevent cartilage resorption, in part, by inhibiting cytokine-induced NO production by chondrocytes and also by inhibiting proteoglycan degradation.

Item Type: Article
Research Institute, Centre or Group: Biomedical Research Centre
Identification Number: 10.1016/j.bcp.2004.11.018
Depositing User: Ann Betterton
Date Deposited: 22 Feb 2008
Last Modified: 09 Dec 2009 18:23
URI: http://shura.shu.ac.uk/id/eprint/421

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